L1900

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

• Synonym(e): Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified
• UNSPSC-Code: 41106200
• NACRES: NA.72

Eigenschaften

• Form: powder
• Methode(n): microbiological culture: suitable
• Anwendung(en): agriculture; microbiology

Beschreibung

Allgemeine Beschreibung

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins.[1] Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Anwendung

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.[2]

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Komponenten

Contains: Tryptone, yeast extract and sodium chloride.

Rekonstituierung

Recommended use at 15.5 g per liter.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable