The mass weight of this product varies from lot to lot and may range from 5 – 25 mg of powder per vial. A brief survey of recent lots indicates an average of approximately 7.0 mg/vial.
Fortfahren mit
K4519
Keratinase
lyophilized powder
Synonym(e):
Keratinase from Bacillus licheniformis, KerA, Keratinolytic protease
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
500 units | Warenkorb auf Verfügbarkeit prüfen | € 415,00 |
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in E. coli BL21
Quality Segment
form
lyophilized powder
specific activity
300-1000 units/mg protein
mol wt
~39 kDa
pH range
5.5-12.5(optimum activity is seen at pH 12.5)
pI
8.73
storage temp.
−20°C
General description
Keratin. Keratinases have also been used for the degradation of prion and prion-like proteins.
Keratinase is a non-specific serine protease that cleaves non-terminal peptide bonds. This enzyme is active between 37°- 70°C. The highest activity is typically seen at 70°C.
Keratinase is activated by 0.10% SDS, 1.0% CTAB, and EDTA. Keratinase is partially inhibited by Tween®20, DMSO, isopropanol, methanol, and ethanol.
Application
Keratinase may be used for enzymatic treatment of elementary body (EB), GAG molecules, and cells in the study of the role glycosaminoglycans (GAGs) in the invasion of host cells by Chlamydia pneumoniae strains.[3]
Biochem/physiol Actions
Keratinase is a particular class of extracellular proteolytic inducible enzyme with the capability of degrading insoluble keratin substrates. [4] It is important for hydrolyzing hair, feather, and collagen in sewage system during waste water treatment. It is also useful in food industry, animal feed preparation etc. Insoluble feather keratin from poultry industry may be converted by enzymatic hydrolysis to glues, feedstuffs, fertilizers, films or used for the production of rare amino acids serine, cysteine and proline.
Preparation Note
The enzyme can be solubilized at 0.5-1.0 mg/mL in either sterile water or phosphate buffer. The best activity is seen with freshly prepared solutions. However, single-use aliquots of Keratinase solutions can be stored at -20° C.
Other Notes
One unit of enzyme is able to hydrolyze casein resulting in an absorbance value as the Folin-Ciocalteau reagent equivalent to 1 umole (181μg) of tyrosine per minute at pH 7.5 at 37 °C.
This enzyme contains a C-terminus 6-Histidine tag.
Legal Information
TWEEN is a registered trademark of Croda International PLC
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Dieser Artikel | |||
|---|---|---|---|
| specific activity 300-1000 units/mg protein | specific activity 7-15 units/mg solid | specific activity ≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid | specific activity ≥7 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form glycerol solution (50%) |
| recombinant expressed in E. coli BL21 | recombinant - | recombinant - | recombinant - |
| mol wt ~39 kDa | mol wt 27 kDa | mol wt 27 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
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