K3393

PDK1, active, His tagged human

Name: PDK1, active, His tagged human
Brand: SIGMA
Price: 514 EUR

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(e):

PDPK1, PRO0461

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Über diesen Artikel

NACRES:

NA.32

UNSPSC Code:

12352200

Specific activity:

102-138 nmol/min·mg

Assay:

≥70% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

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Unterstützung erhalten

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

102-138 nmol/min·mg

mol wt

~67 kDa

UniProt accession no.

Q6A1A2, O15530

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDPK1(5170)

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Dieser Artikel	SRP5247	SRP5003	SRP5018
Sigma-Aldrich K3393 PDK1, active, His tagged human	Sigma-Aldrich SRP5247 PDHK1, active, GST tagged human	Sigma-Aldrich SRP5003 AMPK (α2/β2/γ1), active, His tagged human	Sigma-Aldrich SRP5018 DAPK2, active, GST tagged human
assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
specific activity 102-138 nmol/min·mg	specific activity 1.4-2 nmol/min·mg	specific activity 238-322 nmol/min·mg	specific activity 115-156 nmol/min·mg
Gene Information human ... PDPK1(5170)	Gene Information human ... PDK1(5163)	Gene Information human ... PRKAA2(5563), PRKAB2(5565), PRKAG1(5571)	Gene Information human ... DAPK2(23604)
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

Biochem/physiol Actions

PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns (3, 4, 5) P3 or PtdIns (3, 4) P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns (3, 4, 5) P3 and/or PtdIns (3, 4) P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.

PDPK1 (3-phosphoinositide dependent protein kinase 1) is a serine/threonine protein kinase which is activated upon accumulation of PIP3 (phosphatidylinositol-3,4,5-trisphosphate), a PI3K (phosphoinositide 3-kinase) product. It controls AGC (protein kinase A, G, and C) kinase members, such as AKT (v-akt murine thymoma viral oncogene homolog), p70 ribosomal S6 kinase (S6K), serum- and glucocorticoid-induced protein kinase (SGK), and protein kinase C (PKC) members. It is involved in metabolism, growth, proliferation, and survival.[1][2] PDPK1 is associated with growth of angiosarcoma cells.[3]

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklasse

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

0000447949

SLBQ6165V

SLBJ1182V

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Die Dokumentenbibliothek aufrufen

PDK1 is a potential therapeutic target against angiosarcoma cells.

Makoto Wada et al.

Journal of dermatological science, 78(1), 44-50 (2015-03-03)

Angiosarcoma is a rare and aggressive malignant neoplasm of endothelial cells. Recent studies have shown that the mTOR pathway is also aberrantly activated in cutaneous angiosarcoma. New therapeutic strategies are required because the prognosis of this disease is still poor.

The PDK1 master kinase is over-expressed in acute myeloid leukemia and promotes PKC-mediated survival of leukemic blasts.

Joanna Zabkiewicz et al.

Haematologica, 99(5), 858-864 (2013-12-18)

PDK1 is a master kinase that activates at least six protein kinase groups including AKT, PKC and S6K and is a potential target in the treatment of a range of malignancies. Here we show overexpression of PDK1 in over 40%

PDK1 signaling toward PLK1-MYC activation confers oncogenic transformation, tumor-initiating cell activation, and resistance to mTOR-targeted therapy.

Jing Tan et al.

Cancer discovery, 3(10), 1156-1171 (2013-07-28)

Although 3-phosphoinositide-dependent protein kinase-1 (PDK1) has been predominately linked to the phosphoinositide 3-kinase (PI3K)-AKT pathway, it may also evoke additional signaling outputs to promote tumorigenesis. Here, we report that PDK1 directly induces phosphorylation of Polo-like kinase 1 (PLK1), which in

Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha.

D R Alessi et al.

Current biology : CB, 7(4), 261-269 (1997-04-01)

Protein kinase B (PKB), also known as c-Akt, is activated rapidly when mammalian cells are stimulated with insulin and growth factors, and much of the current interest in this enzyme stems from the observation that it lies 'downstream' of phosphoinositide

PDK1, one of the missing links in insulin signal transduction?

P Cohen et al.

FEBS letters, 410(1), 3-10 (1997-06-23)

The initial steps in insulin signal transduction occur at the plasma membrane and lead to the activation of phosphatidylinositide (PtdIns) 3-kinase and the formation of PtdIns(3,4,5,)P3 in the inner leaflet of the plasma membrane which is then converted to PtdIns(3,4)P2

Global Trade Item Number

SKU	GTIN
K3393-10UG	04061837755972

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