H9034

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD02264926

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• mol wt: 22 kDa (plus 114 kDa galactosidse)
• concentration: ~1 mg/mL
• UniProt accession no.: G9G988, L7S4V3
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: hepatitis C virus ... HCVgp1(951475) ; human ... HBVgp4(944568)

Beschreibung

General description

amino acids 2-192 of HCV polyprotein

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.

Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.

Application

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.

Biochem/physiol Actions

Positive control for HCV antibodies.

Reacts strongly with human HCV positive serum.

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Other Notes

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable