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Merck

H6024

Sigma-Aldrich

Hoechst Stain solution

Synonym(e):

Bisbenzimide H 33258

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352207
PubChem Substanz-ID:

Form

solution

Anwendung(en)

histology

Lagertemp.

2-8°C

SMILES String

O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChIKey

OWRSPPSBNWJJAR-UHFFFAOYSA-N

Allgemeine Beschreibung

Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells. It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.

Anwendung

A fluorescent DNA stain qualified for use in Mycoplama staining.

Komponenten

Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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S A Latt et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(7), 493-505 (1975-07-01)
A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid
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