H5791

Hepatocyte Growth Factor human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): HGF, Hepatopoietin A, Scatter Factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00217422
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 70 kDa (glycosylated)
• Impurities: ≤1 EU/mg

Eigenschaften

• Produktname: Hepatocyte Growth Factor human, HGF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤20 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: monomer 70 kDa (glycosylated)
• packaging: pkg of 5x10 μg, pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• UniProt accession no.: P14210
• storage temp.: −20°C
• Gene Information: human ... HGF(3082)

Beschreibung

General description

Recombinant human hepatocyte growth factor (HGF) is expressed in HEK 293 cells and supplied as a glycosylated lyophilized protein suitable for mammalian cell culture research. HGF is the ligand for the c‑Met receptor and is widely studied in in vitro cell signaling and growth models.

Application

Recombinant HGF has been used in mammalian cell culture applications, including epithelial, endothelial, and hepatocyte‑related in vitro research models.

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that exhibits mitogenic and motogenic activity in vitro and mediates cellular responses through activation of the c‑Met receptor signaling pathway.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable