H4666

Activin A human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): Activin A Protein, Growth Factor, Human Activin A
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD02685750
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 25 kDa (non-glycosylated)
• Impurities: ≤1 EU/mg

Eigenschaften

• Produktname: Activin A human, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤5 ng/mL EC50
• quality: endotoxin tested
• mol wt: dimer 25 kDa (non-glycosylated)
• packaging: pkg of 5x10 μg, pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Activin-A participates in a diverse array of functions that include; hypothalamic/pituitary/gonadal hormone secretion, insulin secretion; cell growth, differentiation and survival (apoptosis); embryonic patterning and development; wound healing; and inflammation/immune response. It was initially identified as a follicle stimulating hormone/FSH-releasing protein (gonadal hormone), Ling N, et al. (1986) and erythroid differentiation factor (EDF), Schwall R, et al. (1989). The FSH activity is linked to an increase in the population of pituitary gonadotrophs by Katayama T, et al. (1990). In addition to gonadotrophs, activin-A also modifies somatotrophs and lactotrophs, Kitaoka, et al. (1988).

Activin-A stimulates glycogenolysis in isolated rat hepatocytes, Mine T ET. al. (1989) and elevates insulin release from rat pancreatic islets, Verspohl EJ et al. (1993). Activin-A stimulates insulin secretion in rat pancreatic islets, Totsuka Y, et al. (1988).

Follistatin, an activin-binding protein, is a principle regulator of activin activity, de Winter JP, et al. (1996); however during embryogenesis, Cripto is an important noncompetitive activin antagonist that facilitates Nodal signaling, Kelber JA, et al. (2008).

A role for activin-A as a regulator of cell proliferation was recognized by Gonzalez-Manchon C and Vale W. (1989); wherein Act-A inhibited the growth of and induced morphological changes in CHO-KI cells in culture, in a way similar to but less potent than TGF-β.

Activin A is involved with the entire process of embryo development from germ cells thru embryonic development to adult tissues. It stimulates spermatogonial proliferation in germ-Sertoli cell cocultures, Mather JP, et al. (1990) and is a maturation factor for oocytes, Itoh M, et al. (1990). Activin A promotes proliferation of human luteinized preovulation granulose cells (ovarian granulose cells), Rabinovici J, et al. (1990).

Analysis Note

The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable