If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
Fortfahren mit
H0887
HEPES -Lösung
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
Synonym(e):
N-(2-Hydroxyethyl)-piperazin-N′-(2-ethansulfonsäure)
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Über diesen Artikel
Produktname
HEPES -Lösung, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
Quality Segment
product line
BioReagent, BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry), dry at room temperature
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
pH
7.0-7.6
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media, suitable for electrophoresis
application(s)
advanced drug delivery
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
Darüber hinaus übertrifft es Bicarbonatpuffer (NaHCO3) in seiner Fähigkeit, den physiologischen pH-Wert trotz Schwankungen der Kohlendioxidkonzentration aufgrund der Zellatmung aufrechtzuerhalten, was ein Schlüsselfaktor für die Aufrechterhaltung der zellulären Homöostase ist. HEPES weist nicht nur eine außergewöhnliche Leistung in der Zellkultur auf, sondern es kann auch in verschiedenen biologischen und biochemischen Studien eingesetzt werden. Es kann auch bei Verfahren wie Immunpräzipitation, Zelllyse und Live-Cell-Imaging nützlich sein, um eine stabile und kontrollierte Umgebung für Experimente zu schaffen. Die Kombination aus Vielseitigkeit, hervorragender pH-Pufferkapazität und minimaler Interaktion mit anderen Molekülen macht HEPES zu einem unverzichtbaren Instrument in verschiedenen Forschungsbereichen.
Application
- Ausgeglichener Salzlösung nach Hanks, Dulbeccos Modifiziertem Eagle Medium und glukosefreiem DMEM, die zur Präparation von Gewebeschnitten eingesetzt werden
- Puffern für an der basolateralen Seite von Kultureinsätzen lokalisierte Leitfähigkeitsmessungen zur Untersuchung der Kationenselektivität
- Zellkulturmedien für die Pufferkapazität, um den pH-Wert der Zelllinie aufrechtzuerhalten
- Puffern bei der Dezellularisierung des Nucleus pulposus vom Schwein
Features and Benefits
- Hochreines Produkt für die biochemische und biologische Forschung
- Sterilfiltriert und auf Zellkulturen getestet
- Gebrauchsfertige Lösung reduziert den Bedarf
- Nützlicher pH-Bereich von 6,8–8,2 und pKa-Wert von 7,5 bei 25 °C
- Gebrauchsfertige Lösung reduziert die Präparationszeit
Preparation Note
Other Notes
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | description BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | description BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | description BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration) |
| assay 0.9—1.1 M | assay ≥99.5% (titration) | assay ≥99.5% (titration) | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable | technique(s) - |
| concentration 1 M | concentration - | concentration - | concentration - |
| product line BioReagent, BioXtra | product line - | product line - | product line BioXtra |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| application(s) advanced drug delivery | application(s) advanced drug delivery | application(s) advanced drug delivery | application(s) diagnostic assay manufacturing |
| assay 0.9—1.1 M | assay ≥99.5% (titration) | assay ≥99.5% (titration) | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable | technique(s) cell culture | mammalian: suitable, competitive inhibition ELISA: suitable | technique(s) - |
| concentration 1 M | concentration - | concentration - | concentration - |
| product line BioReagent, BioXtra | product line - | product line - | product line BioXtra |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| application(s) advanced drug delivery | application(s) advanced drug delivery | application(s) advanced drug delivery | application(s) diagnostic assay manufacturing |
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Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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Hello! Is it dissolved in H2O entirely? or does it have other surfactants?
1 answer-
This product is comprised of HEPES free acid in tissue culture grade water. No other additives or preservatives are present, including surfactants.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Bonjour, Est-ce que ce produit H0887 peut-être congelé à -80°C sans risque de le faire précipiter ?
1 answer-
It is best not to freeze the product as received. However, should the product be inadvertently frozen, it is best to thaw the product slowly. Do not place the frozen product in an incubator or water bath. Once thawed, inspect for any precipitates before use. A clear, colorless solution will be suitable for further use. Should this buffer be required to prepare the stock solution of another reagent that requires freezer storage, thaw slowly if possible.
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