E4913
Plant/Fungi RNA Purification Kit
sufficient for 50 purifications
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NACRES:
NA.52
UNSPSC Code:
41105501
Usage:
sufficient for 50 purifications
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sufficient for 50 purifications
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General description
Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.
Application
For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
- qRT-PCR
- Reverse transcription PCR
- Northern blotting
- RNAse protection
- Primer extension
- Expression array assays
- Next Generation Sequencing
Features and Benefits
- Isolate RNA from a wide range of plant and filamentous fungi samples.
- Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
- Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
- Purification of high yield and quality RNA.
- No phenol/chloroform extraction required.
- Liquid nitrogen is not required for homogenization
Other Notes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
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Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
Lagerklasse
10 - Combustible liquids
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I El-Sharkawy et al.
Journal of experimental botany, 63(3), 1225-1239 (2011-11-15)
Several dwarf plum genotypes (Prunus salicina L.), due to deficiency of unknown gibberellin (GA) signalling, were identified. A cDNA encoding GA 2-oxidase (PslGA2ox), the major gibberellin catabolic enzyme in plants, was cloned and used to screen the GA-deficient hybrids. This
I El-Sharkawy et al.
Journal of experimental botany, 61(6), 1761-1770 (2010-03-06)
Germin-like proteins (GLPs) have several proposed roles in plant development and defence. Two novel genes (Ps-GLP1 and 2) encoding germin-like protein were isolated from plum (Prunus salicina). Their regulation was studied throughout fruit development and during ripening of early and
Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms.
Takao Ito et al.
Archives of virology, 158(1), 273-275 (2012-08-29)
Jocelyn P S Chua et al.
The New phytologist, 196(3), 862-872 (2012-08-31)
Development of the symbiotic association in the bipartite lichen Pseudocyphellaria crocata was investigated by characterizing two regions of the thallus. Thallus organization was examined using microscopy. A HIP1-based differential display technique was modified for use on Nostoc strains, including lichenized
Michaela Novodvorska et al.
BMC genomics, 14, 246-246 (2013-04-13)
Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. The metabolism of storage compounds during conidial germination was also examined and compared to the transcript levels from associated
Protokolle
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.
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