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D2438

Dimethylsulfoxid

sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Dimethyl sulfoxide

Synonym(e):

DMSO, Methylsulfoxid

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Ihnen/SKUVerfügbarkeitPreis
50 mL
Warenkorb auf Verfügbarkeit prüfen
€ 190,00
5 x 10 mL
Warenkorb auf Verfügbarkeit prüfen
€ 196,00

Über diesen Artikel

Lineare Formel:
(CH3)2SO
CAS-Nummer:
Molekulargewicht:
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Grade:
BioPerformance Certified
Technique(s):
cell culture | hybridoma: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

€ 190,00


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grade

BioPerformance Certified

Quality Segment

agency

USP XXIV, meets EP testing specifications, meets USP testing specifications

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

impurities

≤0.1% water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

General description

Dimethylsulfoxid (DMSO) ist ein aprotisches Lösungsmittel. Therapeutische und toxische Moleküle, die nicht wasserlöslich sind, sind oft in DMSO löslich. Flüssiger Stickstoff in Kombination mit einem Gefrierschutzmittel wie DMSO ist ein häufig angewendetes Verfahren zur Konservierung von Zellen. DMSO wird zur Herstellung von Stammlösungen verschiedener Moleküle und auch als Kontrolle für diese Moleküle verwendet. Es wird in Zellmigrationsassays zum Lösen von Zellen nach der Migration verwendet. BPC-getestetes DMSO erfüllt auch die Anforderungen für Anwendungen in der Biotechnologie und Gewebezüchtung.

Application

Dimethylsulfoxid wurde als Vehikelkontrolle zum Kultivieren und Einleiten der Aktivität von hepatischen Sternzellen (HSC) in vivo verwendet.[1][1] Es wurde auch zum Herstellen einer Stammlösung zum Untersuchen der Zytotoxizität von Ferulasäure auf NP-Zellen (Nucleus pulposus) eingesetzt.[2]

Features and Benefits

Gebrauchsfertiges, sterilfiltriertes Produkt, anwendungsfreundlich in Braunglasflaschen mit Schraubkappe mit Septum abgefüllt. Erfüllt die USP- und EP-Testanforderungen

Disclaimer

Unterkühlt leicht und schmilzt langsam bei Raumtemperatur. Das verfestigte Produkt kann durch Erwärmen auf Raumtemperatur wieder verflüssigt werden, ohne dabei geschädigt zu werden.

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Dieser Artikel
4163941640-M34869
technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

GC/GC: suitable

technique(s)

GC/GC: suitable

technique(s)

HPLC: suitable

Quality Level

400

Quality Level

200

Quality Level

-

Quality Level

100

grade

BioPerformance Certified

grade

Molecular Biology

grade

ACS reagent, puriss. p.a.

grade

HPLC grade

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

-

solubility

-

form

liquid

form

liquid

form

liquid

form

liquid

refractive index

n20/D 1.479 (lit.)

refractive index

n20/D 1.479

refractive index

n20/D 1.479

refractive index

n20/D 1.479 (lit.)


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Lagerklasse

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–10 of 17 Questions  
  1. Is it okay to mix it with freezing media that is kept refrigerated? Additionally, when I mixed DMSO with freezing media that was kept refrigerated, a white floating substance appeared. Do you know what might have caused this?

    1 answer
    1. The DMSO is stable at room or refrigerated temperatures. Typically, cell freezing media containing serum and DMSO, are stable for at least several days when thawed and refrigerated. Other constituents of freezing medium, primarily serum, may contribute to the appearance of particulates. For detailed troubleshooting, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further.

      Helpful?

  2. For D2438-5X10ML, do they come in glass ampoule or bottle with lid?

    1 answer
    1. Yes, both package sizes have a septum cap closure as shown in the image below:

      Helpful?

  3. Hi, what is the difference between DMSO product# D2438 and D2650? What is Hybri-Max vs. BioPerformance certified and meets USP testing specification?

    1 answer
    1. Both D2438 and D2650 are suitable for cell culture. The D2650 is part of the Hybri-Max™ product line. Hybri-Max products have been tested for use in hybridoma creation and culture. The D2438 is part of the BioPerformance Certified group of products. These products are tested and optimized for use in biological applications. For this product and a number of others, this includes testing to meet USP and EP guidelines. Please see the links below to review a sample Certificate of Analysis for each item.
      D2650
      https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation
      D2438
      https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation

      Helpful?

  4. Purity grade of the product ?

    1 answer
    1. The purity of this product is listed on the lot specific Certificate of Analysis. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a Certificate:
      https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation

      Helpful?

  5. El DMSO es sensible a la luz, hay que tomar alguna precaución al respecto?

    1 answer
    1. This product is packaged in amber glass and is protected from light. The 10 mL size is packaged in a sealed ampule. Once opened, any content that is not used should also be stored in amber glass. For long term storage, the material may also be stored under inert gas to preserve the shelf life. Note that this material has a tendency to absorb water. Containers should be tightly sealed.

      Helpful?

  6. what are the storing conditions?

    1 answer
    1. This product is stored at room temperature. Store in a well-ventilated place. Keep cool. Please see the Material Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/d2438.

      Helpful?

  7. What is the difference between products D2438 and D8418?

    1 answer
    1. The D2438 is a BioPerformance Certified grade product suitable for cell culture, hybridoma creation, and cryopreservation. It is intended for research use only but does meet USP and EP testing requirements. The D8418 is a molecular biology-grade product suitable for DNA sequencing, PCR, and transfection. It has been tested for RNAse and DNAse. The products can be selected based on the application.

      Please view the sample Certificate of Analysis for each product to see the testing specifications associated with that item:
      https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF734068.pdf
      https://www.sigmaaldrich.com/coa/SIGMA/D2438/RNBL8837

      Helpful?

  8. What concentration of DMSO do I use in a cell freezing media?

    1 answer
    1. DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

      Helpful?

  9. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 17 Questions  

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