CRISPR18V

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

• NACRES: NA.51
• UNSPSC Code: 41106609
• Form: liquid
• Concentration: (1x10⁶ TU/ml (via p24 titering assay))

Eigenschaften

• form: liquid
• Quality Segment: 200
• packaging: vial of 200 μL
• concentration: (1x10⁶ TU/ml (via p24 titering assay))
• application(s): CRISPR
• shipped in: dry ice
• storage temp.: −70°C

Beschreibung

General description

The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Application

Functional Genomics/Screening/Target Validation

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Legal Information

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable