CLLS1049
MCF10A CELLS TP53 (-/-)
human female maMilliporeary glands (Source Disease: Fibrocystic Disease)
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Alle Fotos(1)
About This Item
UNSPSC-Code:
41106514
eCl@ss:
32190102
NACRES:
NA.81
Empfohlene Produkte
product name
MCF10A CELLS TP53 (-/-),
Biologische Quelle
human female mammary glands (Source Disease: Fibrocystic Disease)
OMIM-Hinterlegungsnummer
Lagertemp.
−196°C
Angaben zum Gen
human ... TP53(7157)
Allgemeine Beschreibung
MCF10A CELLS TP53 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
Anwendung
Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.
Leistungsmerkmale und Vorteile
These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 17p13.1
Qualität
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Haftungsausschluss
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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ChenWei Lin et al.
Scientific data, 11(1), 27-27 (2024-01-05)
A wealth of proteogenomic data has been generated using cancer samples to deepen our understanding of the mechanisms of cancer and how biological networks are altered in association with somatic mutation of tumor suppressor genes, such as TP53 and PTEN.
Fenfang Li et al.
Micromachines, 11(5) (2020-05-14)
Deformability is shown to correlate with the invasiveness and metastasis of cancer cells. Recent studies suggest epithelial-to-mesenchymal transition (EMT) might enable cancer metastasis. However, the correlation of EMT with cancer cell deformability has not been well elucidated. Cellular deformability could
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