Fortfahren mit
CLLS1003
DLD1 Cells PIK3CA +/-
human male colon (Source Disease: Colorectal adenocarcinoma)
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 set | Warenkorb auf Verfügbarkeit prüfen | € 4.260,00 |
Über diesen Artikel
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human male colon (Source Disease: Colorectal adenocarcinoma)
Technischer Dienst
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Unterstützung erhaltenProduktname
DLD1 Cells PIK3CA +/-,
biological source
human male colon (Source Disease: Colorectal adenocarcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... PIK3CA(5290)
General description
DLD1 Cells PIK3CA +/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
Application
Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:10
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Features and Benefits
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 3q23.3
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Dieser Artikel | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human male colon (Source Disease: Colorectal adenocarcinoma) | biological source human male colon (Source Disease: Colorectal adenocarcinoma) | biological source human male colon (Source Disease: Colorectal adenocarcinoma) | biological source human male colon (Source Disease: Colorectal adenocarcinoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... PIK3CA(5290) | Gene Information human ... TP53(7157) | Gene Information human ... BAK(578), BAX(581) | Gene Information human ... AKT2(208) |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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