SVCT

94122105, human breast, Epithelial

• Synonym(e): SCTV, SCVT
• UNSPSC-Code: 41106514

Eigenschaften

• product name: SVCT, 94122105, from human breast
• Biologische Quelle: human breast
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

Human breast epithelium, SV40 transformed

Beschreibung der Zelllinie

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

DNA-Profil

Not specified

Nährmedium

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.