AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• UNSPSC Code: 41106514
• Biological source: rat pancreas
• Relevant disease(s): cancer
• Growth mode: Semi-adherent aggregates
• Karyotype: Not specified
• Morphology: Pancreas cells
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: rat pancreas
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 93100618-DNA-5UG, pkg of vial of cells 93100618-1VL
• growth mode: Semi-adherent aggregates
• karyotype: Not specified
• morphology: Pancreas cells
• products: Amylase and exocrine enzymes
• receptors: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

Biochem/physiol Actions

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Rat exocrine pancreatic tumour

Preparation Note

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.