L6.G8

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92102117, rat muscle, Fibroblast

• UNSPSC Code: 41106514
• Biological source: rat muscle
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: rat muscle
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 92102117-DNA-5UG, pkg of vial of cells 92102117-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

A subclone of L6, a rat thigh muscle cell line. Previously, on reaching confluence the cells fused to form myotubes and a twitching movement could be observed. However, these cells have lost the ability to fuse after reaching confluency.

Rat skeletal muscle myoblast

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Cells have lost their ability to fuse after reaching confluency.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.