L-M (TK-)

90083001, mouse connective tissue, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: L-M (TK-), 90083001
• Biologische Quelle: mouse connective tissue
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 45; hyperdiploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C34/An connective tissue

Beschreibung der Zelllinie

Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.

Anwendung

Gene transfer, genetic, biochemical and hybridisation studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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