PC-9 (formerly known as PC-14)

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• Synonym(e): PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent/suspension
• Karyotype: Not specified
• Morphology: Heterogeneous mixture of round cells and spindle shaped cells
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 90071810-DNA-5UG, pkg of vial of cells 90071810-1VL
• growth mode: Adherent/suspension
• karyotype: Not specified
• morphology: Heterogeneous mixture of round cells and spindle shaped cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Biochem/physiol Actions

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Human adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Packaging

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.