ACHN

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human kidney
• Relevant disease(s): metastasis
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human kidney
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 88100508-DNA-5UG, pkg of vial of cells 88100508-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Interferon and interferon-induction studies

Biochem/physiol Actions

Derived from the pleural effusion of a 22 year old male patient with metastatic renal adenocarcinoma. The cells have been reported to be growth inhibited by human interferons.

Human renal adenocarcinoma

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.