THM 7.3.5

Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl), 87120201

• UNSPSC-Code: 12352200

Eigenschaften

• Biologische Quelle: Mouse + Human Transfectoma
• ImmunoDon: Not applicable
• Myelom: J558L
• Reaktivität: Anti-NP (4-hydroxy-3 nitrophenacetyl)
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgM
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse J558L + Mouse VH gene + Human C(μ) gene

Beschreibung der Zelllinie

This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.

Nährmedium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.