AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• Myelom: NS0
• Reaktivität: Application: Fusion partner for hybridoma production
• Methode(n): cell culture | mammalian: suitable
• Isotyp: Not specified
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Horse peripheral blood lymphocytes x Mouse NS0

Beschreibung der Zelllinie

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Immunogen

Influenza A equine 2 virus

Anwendung

Fusion partner for hybridoma production

Nährmedium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Sonstige Hinweise

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