RFL-6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86072501, rat lung, Fibroblast

• UNSPSC Code: 41106514
• Biological source: rat lung
• Growth mode: Adherent
• Karyotype: 2n = 42
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: rat lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 86072501-DNA-5UG, pkg of vial of cells 86072501-1VL
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Derived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.

Rat Sprague-Dawley lung

Preparation Note

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.