OX-97

13082218, from Mouse x Rat Hybridoma

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Rat Hybridoma
• ImmunoDon: Rat PVG spleen
• Myelom: NS1
• Reaktivität: Mouse CD22
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Beschreibung der Zelllinie

The hybridoma cell line OX-97 secretes monoclonal IgG1 antibodies reactive with mouse CD22 domain 2.

Immunogen

Recombinant CD22

Anwendung

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subkultur-Routine

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Sonstige Hinweise

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.