YTS 177.9.6.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 11060819

• UNSPSC Code: 41106514
• Biological source: Rat x Rat Hybridoma
• Technique(s): cell culture | mammalian: suitable
• Myeloma: Y3.AG.1.2.3
• Reactivity: Anti- L3T4 epitope B, mouse CD4

Eigenschaften

• biological source: Rat x Rat Hybridoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 11060819-DNA-5UG, pkg of vial of cells 11060819-1VL
• ImmunoDon: DA rat spleen
• Myeloma: Y3.AG.1.2.3
• Reactivity: Anti- L3T4 epitope B, mouse CD4
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -140 to -196°C

Beschreibung

Immunogen

CBA mouse spleen cells, thymocytes

Application

Rat hybridoma producing antibody against mouse CD4 epitope A

Biochem/physiol Actions

Monoclonal antibody human CD18

Rat hybridoma producing antibody against mouse CD4 epitope A. Cambridge Enterprise Reference number AB111:www.enterprise.cam.ac.uk.

Preparation Note

IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.

Maintain cultures between 3-9 x 10⁵ cells/ml. 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.