328/14

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse nerve (fibroblast)
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal No. varied with passage see reference
• Morphology: Neuronal/fibroblastic

Eigenschaften

• biological source: mouse nerve (fibroblast)
• packaging: tube of 5 μg 08062541-DNA-5UG, pkg of vial of cells 08062541-1VL
• growth mode: Adherent
• karyotype: Modal No. varied with passage see reference
• morphology: Neuronal/fibroblastic
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Beschreibung

Application

Used as a neuronal Model

Biochem/physiol Actions

Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (Sigma Catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (Sigma Catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/14 is of the neuronal type.

Mouse neuroblastoma x mouse L cell fibroblast hybrid

Preparation Note

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].