COS-7-InVitrus

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: African green monkey kidney
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: African green monkey kidney
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 05063001-DNA-5UG, pkg of vial of cells 05063001-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• receptors: Not specified
• technique(s): cell culture: suitable using | mammalian

Beschreibung

Biochem/physiol Actions

COS-7 cell line (Sigma catalog no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.

Monkey African green kidney, SV40 transformed, serum-free

Preparation Note

InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.