Sf21

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: insect ovary
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Spherical
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: insect ovary
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 05022801-DNA-5UG, pkg of vial of cells 05022801-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Spherical
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Protein expression using the Baculovirus vector system

Biochem/physiol Actions

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

Spodoptera frugiperda pupal ovarian tissue

Preparation Note

SF90011 + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.