293 GTP-AC-free

5011003, human kidney (embryonic), Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: 293 GTP-AC-free, 05011003
• Biologische Quelle: human kidney (embryonic)
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Embryo Kidney, serum-free

Beschreibung der Zelllinie

The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the animal component free medium Gene Therapy Medium 3 (Sigma G9916). This cell line grows in clumps in suspension. The original 293 cell line from which this cell line was derived was transformed with sheared human Ad5 DNA.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Nährmedium

Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Subkultur-Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.