Vero/hSLAM

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 4091501, African green monkey kidney, Fibroblast

• Synonym(e): Vero/hSLAM Cells
• UNSPSC Code: 41106514
• Biological source: African green monkey kidney
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: African green monkey kidney
• description: Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 04091501-DNA-5UG, pkg of vial of cells 04091501-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: SLAM; also known as CDw150, a receptor for measles virus
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Monkey African Green Kidney human CDw150 (SLAM) mealses virus receptor expression

Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor′s laboratory.

Preparation Note

MEM with Earle's salts + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) + 0.4 mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.