C9108

Anti-Chk2 antibody, Mouse monoclonal

clone DCS-273, purified from hybridoma cell culture

• Synonym(e): Monoclonal Anti-Chk2 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03457550
• Conjugate: unconjugated
• Clone: DCS-273, monoclonal
• Application: ARR, IHC (p), WB
• Citations: 11

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DCS-273, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 61 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, microarray: suitable, western blot: 2-4 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
• isotype: IgG1
• UniProt accession no.: O96017
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CHEK2(11200)

Beschreibung

Immunogen

recombinant human Chk2.

Application

Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) and for immunoblotting at a concentration of 2 to 4 μg/mL using whole extract of cultured 293T cells.

Biochem/physiol Actions

The epitope recognized by the antibody resides within the FHA domain of the Chk2 molecule. This epitope is likely to be masked by protein-protein interactions in vivo.

The protein propagates signals from damaged or unreplicated DNA. The protein is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.