C7117

Anti-Cullin-1 in Kaninchen hergestellte Antikörper

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Cul1
• MDL-Nummer: MFCD02096156
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 85 kDa
• Speziesreaktivität: human, mouse
• Methode(n): western blot: 0.5-1 μg/mL using whole cell extract of human colon carcinoma HCT-116 cell line and whole cell extract of mouse fibroblasts NIH3T3 cell line
• UniProt-Hinterlegungsnummer: Q13616
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CUL1(8454) ; mouse ... Cul1(26965)

Beschreibung

Allgemeine Beschreibung

Cullin-1 (Cul1) is encoded by the gene mapped to human chromosome 7q36.1. It is a 85 kDa protein that belongs to the family of cullin protein family containing distinct group of mediators (at least eight cullin members Cul1-8).

Immunogen

synthetic peptide located at the C-terminus of human cullin-1 (amino acids 759-776). This sequence is identical in mouse cullin-1 and highly conserved (83% identity) in Drosophila cullin-1.

Anwendung

Anti-Cullin-1 antibody produced in rabbit has been used in western blotting.

Biochem./physiol. Wirkung

Cullin 1(CUL1) is a scaffold protein of the ubiquitin E3 ligase Skp1/Cullin1/Rbx1/F-box protein complex, which ubiquitinates a broad range of proteins that are involved in cell-cycle progression, signal transduction and transcription. Cul1 plays an important role in protein degradation and protein ubiquitination. It regulates breast cancer cell proliferation, migration and invasion. Overexpression of Cul1 leads to breast cancer progression. CUL1 promotes human trophoblast cell invasion and dysregulation of its expression may be associated with pre-eclampsia (PE). It may have some role in rheumatoid arthritis (RA) and its response to methotrexate. Cullin1 may act as an important marker for human gastric cancer lymph node metastasis and prognosis.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable