C4585

Monoklonaler Anti-β-Tubulin-Antikörper

clone TUB 2.1, purified from hybridoma cell culture

• Synonym(e): Monoclonal Anti-β-Tubulin
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: CY3 conjugate
• Clone: TUB 2.1, monoclonal
• Application: IF (d)
• Citations: 38

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: TUB 2.1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 55 kDa
• species reactivity: human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
• storage condition: protect from light
• technique(s): direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) ; mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) ; rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

Beschreibung

General description

Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices.

Immunogen

Tubulin aus Rattenhirn.

Application

Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in:

• immunohistochemical assays
• immunofluorescence
• western blotting
• immunocytochemistry
• immunoblotting
• immunostaining

Biochem/physiol Actions

Der Antikörper erkennt alle fünf Isoformen von β-Tubulin (β1-β5). Er reagiert beim Immunblotting mit den β-Lc- und β-Sc-Fragmenten am Carboxy-Terminus des β−Tubulins. Der Antikörper kann zur Lokalisierung von β−-Tubulin in kultivierten Zellen oder Gewebeschnitten verwendet werden.

Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)