C4396

Kohlensäureanhydrase I aus Humanerythrocyten

• Synonym(e): Carbonat-Dehydratase, Carbonat-Hydro-Lyase
• CAS-Nummer: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.25
• EC Number: 232-576-6
• MDL number: MFCD00081467
• EG-Nummer: 4.2.1.1 (BRENDA, IUBMB)
• Specific activity: 100-500 W-A units/mg protein

Eigenschaften

• form: powder
• Quality Segment: 200
• specific activity: 100-500 W-A units/mg protein
• mol wt: 30 kDa
• color: white
• pI: ~6.6
• UniProt accession no.: P00915
• storage temp.: −20°C
• Gene Information: human ... CA1(759)

Beschreibung

Application

Carbonic anhydrase from human erythrocytes (HCA) has been used to study the molten-globule state of carbonic anhydrase (CA). Chaperone-like α-crystallin binds to this state of the enzyme and prevents its aggregation.[1] The enzyme from sigma has been used for the analysis of thermodynamic stability of the enzyme.[2] Furthermore, its clinical significance has been evaluated in human non-small cell lung cancer.[3]

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in various pathologies such as glaucoma, epilepsy and Parkinson′s disease.[2]

Preparation Note

chromatographically
purified.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable