We bought this product and tried to polarize human PBMC with it. We carefully followed the manufacture’s protocol and tried on different human blood samples. We even used other reagents like monocyte attachment media, detachment media etc. from the same manufacture. We even performed optional step (activation at day 7 with cytokines). However, It didn’t work even on a single sample. We Ana analyzed various M2 markers like CD163, CD206 etc. but we didn’t see a change even in a single marker analyzed. We don’t think that this media works as directed.
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NACRES:
NA.81
UNSPSC Code:
12352207
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Unterstützung erhaltenform
liquid
packaging
pkg of 250 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form aqueous solution |
| packaging pkg of 250 ml | packaging pkg of 250 ml | packaging pkg of 250 ml | packaging pkg of 1 vial |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
General description
M2-Macrophage Generation Medium DXF
Application
Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed.The M2-Macrophage Generation Medium DXF contains M-CSF and allows for the generation of M2-polarized macrophages. This xeno-free medium is ready-to-use and includes cytokines for the directed differentiation of M2-like polarized MDM.Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated
Preparation Note
Basal Medium M2 (250ml)SupplementMix M2 (for 250ml)CytokineMix M2: M-CSF (for 250ml)
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Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Mix at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis Note
All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
Protokolle
Generierung von phagozytischen M1- und M2-Makrophagen aus humanen PBMC innerhalb von 10 Tagen in serum- und xenofreien Zellkulturmedien. Mehr als 350 PromoCell-Produkte entdecken.
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
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