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C1184

Sigma-Aldrich

Cellulase aus Aspergillus niger

greener alternative

powder, ≥0.3 units/mg solid

Synonym(e):

1,4-(1,3:1,4)-β-D-Glucan-4-glucanohydrolase

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About This Item

CAS-Nummer:
9012-54-8
EC-Nummer:
3.2.1.4 (BRENDA, IUBMB)
EG-Nummer:
232-734-4
MDL-Nummer:
MFCD00081510
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

powder

Spezifische Aktivität

≥0.3 units/mg solid

Grünere Alternativprodukt-Eigenschaften

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Grünere Alternativprodukt-Kategorie

, Enabling

Lagertemp.

2-8°C

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Verwandte Kategorien

Allgemeine Beschreibung

The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Cellulase belongs to the family of glycoside hydrolase, which is secreted by various cellulolytic microorganisms.

Anwendung

Cellulase from Sigma has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.
The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.

Biochem./physiol. Wirkung

Cellulase aus Aspergillus niger katalysiert die Hydrolyse von Endo-1,4-β-D-glycosidischen Verbindungen in Zellulose, Lizenin, Gerstenglucan und die Cellooligosacchariden-Cellotriose zu Cellohexaose. Bewirkt keine Spaltung von Cellobiose oder p-Nitrophenyl-β-D-glucosid. Dieses Enzym spaltet auch intaktes Glykosaminoglykan aus einem Kernpeptid durch Hydrolyse der Xylosylserin-Verbindung.

Einheitendefinition

1 U setzt 1.0 μmol Glucose aus Cellulose pro Stunde frei bei pH 5.0 und 37 °C (2 Std Inkubationszeit).

Sonstige Hinweise

Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer

Substrat

Produkt-Nr.
Beschreibung
Preisangaben

M6018

4-Methylumbelliferyl-β-D-cellobiosid, glucanase substrate

C1052

Cellulose Azure, cellulase substrate

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Meena Ganesan et al.
Biotechnology for biofuels, 13, 124-124 (2020-07-21)
The current production of bioethanol based on lignocellulosic biomass (LCB) highly depends on thermostable enzymes and extremophiles owing to less risk of contamination. Thermophilic bacterial cellulases are preferred over fungi due to their higher growth rate, presence of complex multi-enzymes
Fuxi Shi et al.
ACS omega, 5(38), 24780-24789 (2020-10-06)
A major challenge in converting lignocellulose to biofuel is overcoming the resistance of the biomass structure. Herein, sequential dilute acid-alkali/aqueous ammonia treatment was evaluated to enhance enzymatic hydrolysis of poplar biomass by removing hemicellulose first and then removing lignin with
E Schuster et al.
Applied microbiology and biotechnology, 59(4-5), 426-435 (2002-08-13)
Aspergillus niger is one of the most important microorganisms used in biotechnology. It has been in use already for many decades to produce extracellular (food) enzymes and citric acid. In fact, citric acid and many A. niger enzymes are considered
Structural organization and a standardized nomenclature for plant endo-1, 4-beta-glucanases (cellulases) of glycosyl hydrolase family 9
Urbanowicz BR, et al.
Plant Physiology, 144(4), 1693-1696 (2007)
Paulina Tomaszewska et al.
Annals of botany (2021-12-08)
Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment​, often being planted in marginal lands worldwide. We aimed to characterize the nature of their
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Protokolle

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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