For catalog number A8181, we have not studied storage after being diluted with water. We have on the undiluted product a "one year guarantee of performance" for this product from date of shipping to customers.
Fortfahren mit
A8181
Amidoschwarz-Färbelösung 2X
electrophoresis reagent
Synonym(e):
Naphtholblauschwarz -Lösung, Amidoschwarz 10B
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 ea | Warenkorb auf Verfügbarkeit prüfen | € 209,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C22H14N6Na2O9S2
CAS-Nummer:
Molekulargewicht:
616.49
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5374263
€ 209,00
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Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
liquid
SMILES string
[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key
AOMZHDJXSYHPKS-DROYEMJCSA-L
General description
Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.
Application
Amido Black Staining Solution 2X has been used in the staining:
- serum proteins present in the culture supernatant[1]
- cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
- histones from chromatin samples
Preparation Note
Nach dem Verdünnen mit destilliertem Wasser auf ein Endvolumen von 500 mL, enthält die Lösung 0.1% (w/v) Amidoschwarz, 25% (v/v) Isopropanol und 10% (v/v) Essigsäure.
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| form liquid | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Lagerklasse
3 - Flammable liquids
wgk
WGK 1
flash_point_f
53.6 °F
flash_point_c
12 °C
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can this reagent be stored for an extended period of time after being diluted to 1x with water?
1 answer-
Helpful?
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Could you please provide the staining procedure and destaining formulation for the Amino Black Staining solution 2x that is suitable for staining in 2-40% gels?
1 answer-
To detect total protein on a Western blot, follow these steps:
1.Prepare Amido Black 1X Staining Solution by diluting Amido Black Staining Solution (Product Number A8181) 1:1 with deionized water.
2. After transferring proteins onto nitrocellulose membrane, immerse membrane in sufficient Amido Black 1X Staining Solution to cover. Stain for 1 minute.
3. Destain the membrane by placing in an aqueous solution containing 25% isopropanol and 10% acetic acid for 30 minutes. If a lighter background is desired, destain overnight.
4. Store the destained membrane in deionized water or allow to air dry.
Product Number A8181 Store at Room Temperature Product Description: Amido Black staining solution is used for the detection of microgram quantities of proteins transferred to nitrocellulose membranes. In comparison to staining with Brilliant Blue R, staining with Amido Black results in significantly less background on nitrocellulose membranes. Because nitrocellulose membranes are more stable in isopropanol than in methanol or other solvents, the formulation contains isopropanol. After a 1:1 dilution with deionized water, Amido Black Staining Solution contains: 0.1% Amido Black (w/v), 25% Isopropanol (v/v), 10% Acetic Acid (v/v). Note: Not suitable for use with charge modified nylon membranes.Helpful?
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