Biological dyes do not ship with protocols for use. There are published procedures for Sudan Black B for cytology that provide a formulation using Phosphate-buffered Phenol. The Atlas of CytoChemistry and Immunohistochemistry of Hematologic Neoplasms by Sun, Li and Yam, ASCP Press, 1985 gives the following formulation:
Dissolve 32 grams of crystal Phenol in 60 ml of absolute Ethanol. Once dissolved, prepare the Phosphate buffer solution. Add 0.6 grams Sodium Phosphate, then add distilled water until the volume reaches 200ml.
The instructions then state to prepare the working solution by adding 60 ml of the alcoholic Sudan Black B stock solution to 40 ml of the Phenol Phosphate buffer.
Fortfahren mit
199664
Sudanschwarz B
certified by the BSC
Synonym(e):
Ceresschwarz BN, Fettschwarz BN, Neptunschwarz, Solvent Black 3
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 g | Warenkorb auf Verfügbarkeit prüfen | € 95,10 |
Über diesen Artikel
Empirische Formel (Hill-System):
C29H24N6
CAS-Nummer:
Molekulargewicht:
456.54
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
224-087-1
MDL number:
Beilstein/REAXYS Number:
723248
Colour Index Number:
26150
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenagency
certified by the BSC
Quality Segment
form
powder
technique(s)
microbe id | staining: suitable
color
dark brown to very dark brown
mp
120-124 °C (lit.)
λmax
415 nm (2nd), 598 nm
ε (extinction coefficient)
≥20000 at 596-603 nm in ethanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
InChI
1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
InChI key
YCUVUDODLRLVIC-VPHDGDOJSA-N
Application
Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.
Zertifiziert für die Fettfärbung nach der Churukian Variante des Lillie und Asburn-Verfahrens ("supersaturated isopropanol method"). Sudan III ersetzt Ölrot O in dem Verfahren. Burdon-Färbung für bakterielle Lipide.
Biochem/physiol Actions
Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description certified by the BSC | description Dye content 98 % | description Certified Biological Stain Commission,Dye content ≥80 % | description certified by the BSC, BioXtra |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - |
| color dark brown to very dark brown | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder or crystals | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Muta. 2
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
How to dissolve Sudan Black B for staining cytology bone marrow smears?
1 answer-
Helpful?
-
-
Can Sudan black used for Immunofluorescence specifically for paraffinized eyeball sectioning?
1 answer-
Sudan Black B is not a fluorescent dye. Sudan Black B has been used to block autofluorescence. The following information is available from the website. Although the reference below is specific to brain tissue, the expectation is that Sudan Black B should be able to be used for a variety of different tissue types. There is no specific information available for paraffin sections of eyeballs.
Sudan Black B treatment reduces autofluorescence and improves the resolution of in situ hybridization-specific fluorescent signals of brain sections.
Histology and histopathology (2010-06-17)
V C Oliveira, R C V Carrara, D L C Simoes, F P Saggioro, C G Carlotti, D T Covas, L Neder
PMID20552552
ABSTRACT
Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels.Helpful?
-
-
How to dissolve Sudan Black B for staining tissue?
1 answer-
Sudan Black B can be dissolved in a variety of different solvents depending upon the intended use. Dissolve 0.7 grams of Sudan Black B in 100 ml of propylene glycol. The solution should be heated to 100° C for a few minutes, stirred constantly, and then filtered for optimal results.
Helpful?
-