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Merck

A2230

Sigma-Aldrich

Apyrase aus Kartoffeln

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonym(e):

Adenosin-5′-diphosphatase, Adenosin-5′-triphosphatase und Adenosin-5′-diphosphatase.

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100 UNITS
€ 416,00

€ 416,00


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100 UNITS
€ 416,00

About This Item

CAS-Nummer:
EC-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

€ 416,00


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Biologische Quelle

potato

Form

lyophilized powder

Qualität

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

Zusammensetzung

protein, ≥30%

Fremdaktivität

Acid Phosphatase ≤2% of base activity

Versandbedingung

wet ice

Lagertemp.

−20°C

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Anwendung

Mindestens zwei Isoenzyme kommen in verschiedenen Varietäten von S. tuberosum vor: eines mit einem hohen ATPase/ADPase-Verhältnis (∼10) und ein weiteres mit einem niedrigen Verhältnis (∼1).
Reaktion: ATP → ADP+Pi → AMP+2Pi.
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1]. Product A2230 is a high activity apyrase.

Biochem./physiol. Wirkung

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Verpackung

Sold on the basis of ATPase units.

Einheitendefinition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physikalische Form

Lyophilized powder containing potassium succinate buffer salts.

Angaben zur Herstellung

Derived from red potato

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Xing Liu et al.
Plant physiology, 160(4), 1985-1995 (2012-10-17)
Recent evidence indicates that extracellular nucleotides regulate plant growth. Exogenous ATP has been shown to block auxin transport and gravitropic growth in primary roots of Arabidopsis (Arabidopsis thaliana). Cells limit the concentration of extracellular ATP in part through the activity
Martina Nowak-Machen et al.
American journal of respiratory cell and molecular biology, 48(5), 601-609 (2013-01-26)
Critically ill patients are routinely exposed to high concentrations of supplemental oxygen for prolonged periods of time, which can be life-saving in the short term, but such exposure also causes severe lung injury and increases mortality. To address this therapeutic
Joanne S J Chia et al.
Journal of biomedicine & biotechnology, 2012, 320495-320495 (2012-11-03)
Diabetes mellitus encompasses two distinct disease processes: autoimmune Type 1 (T1D) and nonimmune Type 2 (T2D) diabetes. Despite the disparate aetiologies, the disease phenotype of hyperglycemia and the associated complications are similar. In this paper, we discuss the role of

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