A5763

Adenosine 3′,5′-diphosphate disodium salt

≥96%

• Synonym(e): 3′-phosphoadenosine 5′-phosphate, 3′-phosphorylated nucleotide, PAP, 3′-Phosphoadenosine 5′-phosphate
• Empirische Formel (Hill-System): C₁₀H₁₃N₅Na₂O₁₀P₂
• CAS-Nummer: 75431-54-8
• Molekulargewicht: 471.16
• MDL-Nummer: MFCD00057007
• UNSPSC-Code: 41106305
• eCl@ss: 32160414
• PubChem Substanz-ID: 24891020
• NACRES: NA.51

Eigenschaften

• Biologische Quelle: synthetic (inorganic)
• Qualitätsniveau: 200
• Assay: ≥96%
• Form: powder
• Löslichkeit: water: 25 mg/mL, clear, colorless to very faintly yellow
• Lagertemp.: −20°C
• SMILES String: [Na].Nc1ncnc2n(cnc12)C3OC(COP(O)(O)=O)C(OP(O)(O)=O)C3O
• InChI: 1S/C10H15N5O10P2.Na.H/c11-8-5-9(13-2-12-8)15(3-14-5)10-6(16)7(25-27(20,21)22)4(24-10)1-23-26(17,18)19;;/h2-4,6-7,10,16H,1H2,(H2,11,12,13)(H2,17,18,19)(H2,20,21,22);;
• InChIKey: ISROZYFZEAVMSP-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

3′-phosphoadenosine 5′-phosphate (PAP), a 3′-phosphorylated nucleotide is found in almost all organisms and is obtained as a by-product of sulfur and lipid metabolism.

Anwendung

Adenosine 3′,5′-diphosphate disodium salt has been used:

• to spot sample on cellulose high-performance thin-layer chromatography (HPTLC) plates in two-dimensional thin layer chromatography
• in enzyme activity assay to study the activities of HOS2/FIERY1 wild type
• hos2 mutant and fiery1?2 mutant protein against 3′-phosphoadenosine 5′-phosphate (PAP)
• as a standard for the quantification of phosphoadenosines

Biochem./physiol. Wirkung

3′-phosphoadenosine 5′-phosphate (PAP) is capable of blocking exoribonucleases (XRNs) activity in the nucleus and cytosol. It stimulates stomatal closure and can serve as a secondary messenger during abscisic acid (ABA) signaling. It is capable of blocking RNA catabolism. Hence it may serve as a physiological modulator of poly (ADP-ribose) polymerase 1 (PARP1) activity.

Adenosine 3′,5′-diphosphate (PAP) is used to study the kinetics and mechanisms of hydroxysteroid sulfotransferases such as SULT1A1, SULT2A1 of which it is product inhibitor.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H315,H319,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves