76546

Lipase, immobilisiert auf Immobead 150 aus Thermomyces lanuginosus

≥3000 U/g

• Synonym(e): Immobilized lipase, Lipase enzyme
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥3000 U/g
• Biological source: fungus (Thermomyces lanuginosus)

Eigenschaften

• biological source: fungus (Thermomyces lanuginosus)
• form: powder (or beads)
• specific activity: ≥3000 U/g
• technique(s): analytical sample preparation: suitable
• color: white to off-white
• application(s): life science and biopharma
• storage temp.: 2-8°C
• Quality Level: 100

Beschreibung

General description

Research area: Cell Signaling

Lipase from Thermomyces lanuginosus (TLL) is a basophilic, single-chain protein and thermostable enzyme. It belongs to the alpha/beta-hydrolase fold family. TLL is roughly spherical and is made up of a central seven-stranded β-parallel sheet with five interconnecting α-helices.[1]

Application

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus has been used:

• as a biocatalyst to study ultrasound-assisted transesterification of a blend of non-edible oils with methanol.
• as a catalyst in the synthesis of medium chain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) rich oils by enzymatic structuring of flax seed oil.
• for the resolution of chiral compounds and the transesterification production of biodiesel.
• to enhance the efficiency and selectivity of the enzymatic synthesis of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester. [2]
• to facilitate a chemoenzymatic process for the continuous synthesis of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol, a crucial intermediate for Eribulin synthesis, within a packed-bed reactor setup.[3]

Biochem/physiol Actions

It participates in catalyzing the hydrolysis of triglycerides at the water-lipid interface and produces mono-, di-glycerides, and free fatty acids. Due to its versatility, this enzyme is applicable in various industries including, detergents, paper, leather, baking, fine chemicals, fuel, and biocatalysts.[1]

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 10.0 und 40°C freisetzt (Tributyrin, Fluka^®-Nr. 91010, als Substrat)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)