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4381664

iTRAQ® Reagent - Multiplex Buffer Kit

Synonym(e):

isobaric tags for relative and absolute quantitation, buffer kit 

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Über diesen Artikel

NACRES:
NA.25
UNSPSC Code:
12161503

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shipped in

dry ice

storage temp.

−20°C

General description

Contains SDS – Denaturant, TCEP Reducing Reagent, MMTS Cysteine Blocking Reagent, TEAB Buffer and Isopropanol, sufficient material for either two 8-plex Multiplex Kits, three 4-plex Kits, or four mTRAQ® 10 Assay Kits.
The iTRAQ® Reagents are a set of isobaric, multiplexed, amine-specific, stable-isotope reagents that can label all peptides[1] in up to eight different biological samples at the same time, allowing for simultaneous identification and quantitation, both relative and absolute, while preserving important post-translational modification (PTM) information. The iTRAQ® reagents can be used to study the differential expression of proteins in perturbed systems.[1]

Application

iTRAQ® Reagent - Multiplex Buffer Kit has been used in the isobaric tag for relative and absolute quantitation (iTRAQ) labeling of protein samples.[2][3][4]

Analysis Note

To view the Chemistry Quick Reference Card for the iTRAQ® Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

MTraq is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.

pictograms

FlameExclamation mark

signalword

Danger

target_organs

Central nervous system

Lagerklasse

3 - Flammable liquids

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

wgk

WGK 3

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3


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Hongmei Lu et al.
Clinical proteomics, 16, 33-33 (2019-08-07)
Diabetic nephropathy is the most frequent cause of end-stage renal disease worldwide. Identification of biomarkers for diabetic nephropathy for early diagnosis may be the key to avoiding damage from this condition. Proteomic iTRAQ technology was first used to identify differentially
Lynn R Zieske
Journal of experimental botany, 57(7), 1501-1508 (2006-04-01)
Proteomic research includes the characterization of protein mixtures in order to understand complex biological systems and determine relationships between proteins, their function, and protein-protein interactions. Often the goal of such research is to monitor changes of proteins in perturbed systems
Kening Li et al.
Evidence-based complementary and alternative medicine : eCAM, 2019, 5107198-5107198 (2019-06-22)
NaoMaiTong (NMT) is widely used in the treatment of cerebral ischemia but the molecular details of its beneficial effects remain poorly characterized. In this study, we used iTRAQ using 2D LC-MS/MS technology to investigate the cellular mechanisms governing the protective
Xiangyu Deng et al.
MicrobiologyOpen, 9(5), e1012-e1012 (2020-02-29)
β-Glucosidase production by Aspergillus niger is accompanied by an inevitable temperature increase in the industrial fermentation environment. Hence, the synthetic process of β-glucosidase is negatively affected. However, our understanding of the heat stress response (HSR) mechanism in A. niger is still
Juan Du et al.
FEBS letters, 592(22), 3736-3749 (2018-10-13)
Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome-wide analysis of protein oscillations is still lacking in Drosophila. In this study, the total protein and phosphorylated protein in Drosophila heads in a 24-h daily time-course

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