12080201

L5178Y tk^+/- 3.7.2c (IVGT)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., from mouse thymus

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: mouse thymus
• Growth mode: Suspension
• Morphology: Small rounded cells
• Technique(s): cell culture | mammalian: suitable

Eigenschaften

• biological source: mouse thymus
• Quality Segment: 100
• description: Mouse DBA/2 lymphoma L5178Y tk^+/-
• packaging: tube of 5 μg 12080201-DNA-5UG, pkg of vial of cells 12080201-1VL
• growth mode: Suspension
• morphology: Small rounded cells
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

The L5178Y tk^+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

Fischer′s medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS)

Maintain cultures in logarithmic growth phase between 3 x 10⁴ and 1 x 10⁶ cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Sicherheitshinweise

• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Lagerklasse: 12 - Non Combustible Liquids