After the discontinous density gradient is prepared using Histopaque 10771 and Histopaque 1119, the tubes are centrifuged for 30 minutes at 700G. The Product Information Sheet is available from the website and includes the complete recommended procedure.
11191
Histopaque®-1119
sterile-filtered, density: 1.119
Histopaque®-1119
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100 ML
€ 165,00
6 X 100 ML
€ 749,00
Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12352207
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Unterstützung erhaltensterility
sterile-filtered
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.119
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| density 1.119 | density 1.077 | density 1.083 | density 1.077 g/mL |
| form liquid | form liquid | form liquid | form solution |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility aseptically filled |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
Histopaque®-1119 ist ein steriles, endotoxingeprüftes, gebrauchsfertiges Medium. Die Lösung besteht aus Polysaccharose und Natriumdiatrizoat, eingestellt auf eine Dichte von 1,119 g/ml und einen pH-Wert von 8,8 bis 9,0.
Application
Histopaque®-1119 eignet sich zur Verwendung in Kombination mit Histopaque-1077, da es die schnelle Rückgewinnung von lebensfähigen mononukleären Zellen und Granulozyten aus kleinen Mengen Vollblut oder Knochenmark ermöglicht.
Biochem/physiol Actions
Der Prozess umfasst das Bilden eines Doppelgradienten beim Schichten eines gleichen Volumens Histopaque-1077 über Histopaque-1119. Antikoaguliertes Vollblut wird vorsichtig auf das obere Histopaque-1077-Medium geschichtet. Beim Zentrifugieren werden Erythrozyten durch Polysaccharose aggregiert und setzen sich dadurch schnell ab. Granulozyten kommen an der unteren Histopaque-1077/1119-Grenzfläche vor, wogegen Lymphozyten und andere mononukleäre Zellen an der oberen Plasma/Histopaque-1077-Grenzfläche vorkommen. Die meisten unerheblichen Thrombozyten, die in der oberen Grenzfläche vorkommen, werden durch Zentrifugieren bei langsamer Geschwindigkeit während Waschschritten entfernt. Die Erythrozyten-Kontaminierung ist vernachlässigbar.
Features and Benefits
Histopaque®-1119 bietet zahlreiche Vorteile, z. B.:
- optimale Rückgewinnung von lebensfähigen Zellen
- selektive Trennung der Blutzelltypen
- keine Zellverformungen
- Lebensfähigkeit der Zellen bleibt erhalten
- minimale Interferenz durch Fremdzellen
- reproduzierbare Leistung von Charge zu Charge
- sterilfiltriert und endotoxingeprüft
- vor Licht geschützt bei 2–8 °C mindestens 2 Jahre stabil
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Beschreibung
Preisangaben
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Lagerklasse
10 - Combustible liquids
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Protokolle
Ficoll®-400 ist ein hochverzweigtes Polymer, das durch Copolymerisation von Saccharose und Epichlorhydrin gebildet wird. Ficoll® 400 ist vollständig nicht-ionisch. Aufgrund der großen Anzahl an Hydroxylgruppen ist Ficoll® 400 sehr hydrophil und extrem wasserlöslich.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Verwandter Inhalt
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Bei wieviel g muss das geschichtete (1,1077/1,119/Blut) und wie lange zentrufugiert werden um eine Trennung zu erreichen?
1 answer-
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 answer-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
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Is there a product similar to Mono-Poly resolving media?
1 answer-
The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 answer-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 answer-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 answer-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Helpful?
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When using Product 11191, Histopaque®-1119, what is the expected percent recovery for the band containing neutrophils.
1 answer-
No percent recovery data has been generated. Due to the importance of technique with the Histopaque 1119-1, percent recovery varies with the skill of the operator.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
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No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 answer-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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