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B0126

Bromphenolblau

Powder

Synonym(e):

3′,3″,5′,5″-Tetrabrom-phenolsulfonphthalein, BPB, Bromphenolblau, Sulton Form

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Größe/SKUVerfügbarkeitPreis
25 g
Warenkorb auf Verfügbarkeit prüfen
€ 114,00

Über diesen Artikel

Empirische Formel (Hill-System):
C19H10Br4O5S
CAS-Nummer:
Molekulargewicht:
669.96
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
204-086-2
MDL number:
Beilstein/REAXYS Number:
61698

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Produktname

Bromphenolblau, titration: suitable

Quality Segment

agency

suitable for SM 2310, suitable for SM 2320

form

powder

technique(s)

titration: suitable

color

faint beige to very dark beige, faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

solubility

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

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Dieser Artikel
114391B802695290
description

titration: suitable

description

ACS reagent

description

Molecular Biology, suitable for electrophoresis

description

suitable for microscopy

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

electrophoresis: suitable

technique(s)

microbe id | staining: suitable

color

faint orange to very dark orange, faint beige to very dark beige

color

-

color

-

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder or crystals

solubility

methanol: 10 mg/mL

solubility

ethanol: 0.1%

solubility

-

solubility

water: 0.01 g/10 mL, blue to very deep blue

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. Is it safe to use bleach as a deactivating agent with B0126, considering that bleach is an oxidizing agent?

    1 answer
    1. B0126 is a pH indicator that changes color based on pH levels. It turns blue at pH 4.6 and above, yellow below pH 3.0, and may turn green between pH 4.6 and 3.0. A change to a color not mentioned above or the solution becoming clear indicates the presence of bleach residues.

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