63016

M-17-Agar

NutriSelect^® Basic, powder, for Lactococcus spp., for Streptococcus spp., pack of 500 g, suitable for microbiology

• Synonym(e): M17 Lactococcal Agar
• NACRES: NA.74
• UNSPSC Code: 41171605
• Manufacturer/tradename: NutriSelect^® Basic
• Form: powder
• Suitability: Lactococcus spp., Streptococcus spp.
• Application(s): agriculture; environmental; food and beverages, microbiology

Eigenschaften

• sterility: non-sterile
• Quality Segment: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 12.75 g/L , ascorbic acid, 0.5 g/L , casein peptone (tryptic), 2.5 g/L , disodium β-glycerophosphate pentahydrate, 19 g/L , magnesium sulfate hydrate, 0.25 g/L , meat extract, 5 g/L , meat peptone (peptic), 2.5 g/L , soyapeptone (papainic), 5 g/L , yeast extract, 2.5 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 7.1±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages, microbiology
• suitability: Lactococcus spp., Streptococcus spp.

Beschreibung

General description

M-17 Agar is a specialized culture medium designed to support the growth of lactic streptococci. It contains casein peptone, meat peptone, soya peptone, yeast extract, and meat extract, which provide carbonaceous and nitrogenous compounds, vitamin B complex, and other essential growth factors. Ascorbic acid stimulates the growth of lactic streptococci, while magnesium sulfate supplies essential ions. Disodium β-glycerophosphate pentahydrate enhances the buffering capacity of the medium, promoting the growth of lactic streptococci and selectively inhibiting lactobacilli. The medium can also be used to detect phage infection by the presence of large, distinct plaques in the host bacteria’s growth, but plaque assays require the absence of glycerophosphate, which prevents calcium precipitation necessary for bacteriophage activity.

Application

M-17 Agar media is proposed for the cultivation, detection and enumeration of Iactic streptococci in milk and dairy products. The M-17 media are superior to other comparable culture media for the cultivation of the fastidious species Streptococcus cremoris, Streptococcus diacetilactis and Streptococcus Iactis. It is also suitable for cultivation and maintenance of starter cultures for cheese and yogurt manufacturing. This medium helps in detecting streptococcal mutants which are lactose non-fermenters. M-17 Agar media may also be used for the differentiation of bacteriophages infecting lactic streptococci.

Features and Benefits

NutriSelect^®Basic is a powdered culture media offering

• Qualitycontrol with growth promotion test according to industry standards
• Qualityassurance acc. to ISO 9001
• Cost-efficient powderedmedia

Preparation Note

Dissolve 50 g in 950 ml distilled water and sterilize by autoclaving at 121°C for 15 minutes. After cooling add 50 ml aseptic 10% lactose solution.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.15108 - M 17 agar acc. to TERZAGHI

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)