There are no protocols for using Toluidine Blue O to stain MMA sections.
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Wichtige Dokumente
198161
Toluidinblau O
certified by the BSC
Synonym(e):
Bluten-chlorid, Methylen Blau T50 oder T extra, Rhodulinreinblau 17G, Tolonium-chlorid
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5 G
€ 127,00
25 G
€ 444,00
About This Item
Empirische Formel (Hill-System):
C15H16ClN3S
CAS-Nummer:
Molekulargewicht:
305.83
Farbindexnummer:
52040
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47
Empfohlene Produkte
Agentur
certified by the BSC
Qualitätsniveau
Form
powder
Zusammensetzung
Dye content, 80%
Farbe
dark green
λmax
626 nm
ε (Extinktionskoeffizient)
≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O
Anwendung(en)
diagnostic assay manufacturing
hematology
histology
Lagertemp.
room temp
SMILES String
[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
InChI
1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
InChIKey
GEDVVYWLPUPJJZ-UHFFFAOYSA-N
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Allgemeine Beschreibung
Ein metachromatischer Farbstoff geeignet für eine Vielzahl von histologischen Färbungen.
Anwendung
Biochem./physiol. Wirkung
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls.[3] TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite.[4] TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries.[5] This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.[6]
Eignung
Zertifiziert für den Einsatz in der metachromatischen Färbung von Mastzellen und Knorpel nach der Churukian-Schenk-Methode und der Nocht-Methode für Paraffinschnitte.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue O-mediated lethal photosensitization
komerik N
Journal of Applied Microbiology, 92(4), 618?623-618?623 (2002)
A TRIBASIC STAIN FOR THIN SECTIONS OF PLASTIC-EMBEDDED, OSO4-FIXED TISSUES.
P M GRIMLEY
Stain technology, 39, 229-233 (1964-07-01)
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There is growing evidence that the quality of spermatozoa decreases with age and that children of older fathers have a higher incidence of birth defects and genetic mutations. The free radical theory of aging proposes that changes with aging are
The Effect of Variable Energy Input from a Novel Light Source on the Photoactivated Bactericidal Action of Toluidine Blue O on Streptococcus mutans
Williams J A
Caries Research, 37, 190?193-190?193 (2003)
The adsorption kinetics and removal of cationic dye, Toluidine Blue O, from aqueous solution with Turkish zeolite
Alpat S K
Journal of Hazardous Materials, 151(1), 213-220 (2008)
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Could you provide the protocol for using Toluidine Blue for MMA sections?
1 answer-
Helpful?
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Is there a recommendation for dissolving Toluidine Blue (198161-5g lot MKCK0257) at 1 mg/ml in water?
1 answer-
This product is released based on the testing performed by the Biological Stain Commission. There is no specific solubility specification for this product. At the BSC, the product is dissolved at 50 mg in 250 ml of water for one test, and 100 mg of the dye is dissolved in 150 ml of water for the second test, followed by the addition of additional alcohol. When used to stain frozen sections, a 0.5% solution is typically prepared in 20% alcohol. Some procedures call for dissolving at 10 mg/ml, but this amount of dye cannot always be dissolved. Once dissolved, the solution should be relatively stable.This product does not appear to be hygroscopic, and its solubility can vary somewhat from lot to lot.
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