The recommendation to not vortex these solutions is because it would introduce dissolved oxygen into the solution which may oxidize the luminol. Over time this would shorten the shelf life of the reagents.
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technique(s)
western blot: suitable
detection method
chemiluminescent
shipped in
ambient
General description
Application
Ausreichend für 1.000 cm²
Preparation Note
Other Notes
Lösung B- 50 ml
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| description Western blotting, HRP | description Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents. | description Western blotting, dot/slot blotting | description 100 ml TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble) for ELISA & Immunohistochemistry. |
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) dot blot: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable, ELISA: suitable |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent | detection method - |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in wet ice |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_c
Not applicable
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Why does the protocol say not to agitate the mixed working solution? What is the rationale or reasoning behind this?
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