Yes, the Amicons can tolerate up to 0.5 M NaOH.
UFC9050
Amicon® Ultra Zentrifugenfilter, 50 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 50 kDa
Synonym(e):
15 ml Probenvolumen, 50 kDa, MWCO, Zentrifugenkonzentrator
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
8 pkg | Warenkorb auf Verfügbarkeit prüfen | € 156,00 |
24 pkg | Warenkorb auf Verfügbarkeit prüfen | € 414,00 |
96 pkg | Warenkorb auf Verfügbarkeit prüfen | € 1.530,00 |
Über diesen Artikel
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
50 kDa MWCO
Working volume:
≤15 mL , 15 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
Technischer Dienst
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Unterstützung erhaltenQuality Segment
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL , 15 mL
pore size
50 kDa MWCO
greener alternative category
shipped in
ambient
General description
Filtertyp: Ultrafiltration
Wir möchten Ihnen umweltverträglichere Alternativprodukte für nachhaltigere Forschungslösungen anbieten. Die innere Filtrationsvorrichtung besteht zu 60 % aus biozirkulärem Material, das auf der Grundlage des ISCC-Massenbilanzierungsverfahrens verwendet wird. Weitere Informationen finden Sie hier .
Application
- Ultrafiltration mit einer Ultracel®-Membran mit 50.000-Dalton-Molekulargewichtsausschlussgrenze.
- Konzentration von biologischen Proben, die Antigene, Antikörper, Enzyme, Nukleinsäuren oder Mikroorganismen enthalten.
- Aufreinigung von makromolekularen Komponenten aus Gewebekulturextrakten oder Zelllysaten und Proteinentfernung vor der HPLS.
- Konzentration von verdünnten oder vor-aufgereinigten Proteinen aus Säuleneluenten.
- Entsalzung, Pufferaustausch und Proteindialyse.
Features and Benefits
- Ultracel® regenerierte Cellulosemembran mit 50-kDa-NMWCO ermöglicht eine Retentatrückgewinnung von mehr als 90 %.
- Die vertikale Membran verringert die Konzentrationspolarisation für ultraschnelle Rotationszeiten (innerhalb 10–15 Minuten).
- Die heißversiegelte Membran minimiert die Downstream-Extractables.
- 100 % integritätsgeprüft für eine verlässliche Leistung.
- Praktische Probenüberwachung mit einem transparenten Gehäuse und Skaleneinteilung für das Volumen.
- Direkter Probenzugang für die Pipettiervorrichtung eliminiert den Verarbeitungsschritt, um das Konzentrat zu gewinnen.
- Hohe Konzentrationsfaktoren von 80–100 X.
Physical form
Farbcode: Transparent mit rosafarbener Kappe
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dieser Artikel | |||
|---|---|---|---|
| parameter 15 mL sample volume | parameter 2 mL sample volume | parameter 4 mL sample volume | parameter 0.5 mL sample volume |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| working volume ≤15 mL | working volume ≤2 mL | working volume ≤4 mL | working volume ≤500 μL , 0.5 mL |
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Lagerklasse
11 - Combustible Solids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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is Amicon® Ultra Centrifugal Filter, 50 kDa MWCO compatible with 0.5 M NaOH washing?
1 answer-
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Hello, is Amicon® Ultra Centrifugal Filter, 50 kDa MWCO sterile? I'd like to use it for buffer exchange of Trastuzumab.
1 answer-
Amicon®Ultra-15 devices are supplied non-sterile and are for single use only.
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Hello, I want to buffer exchange my antibody (150 KDa) samples using a 15mL, 50KDa MWCO centrifugal filter and a swing bucket centrifuge. What will be the recovery or will there be any protein loss?
1 answer-
It is impossible to predict recovery as it depends on the sample load and running conditions. Protein loss in the concentrate may occur due to adsorptive losses, over-concentration, or the passage of the sample through the membrane. For optimal retention, it is generally recommended to select a membrane with a molecular weight cut-off (MWCO) that is at least 2 times smaller than the smallest target molecule.
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Can this filter system block large molecules like lipids, proteins, sugars, or starch and allow small molecules to pass through like water, ethyl alcohol, ethyl acetate, and vanillin.
1 answer-
The Amicon Ultra products are designed to retain, not filter, and allow high recovery of smaller molecules. For example, let's say you have a 120 kDa molecule and you use a 50 kDa mwco filter in the Amicon Ultra. Most large molecules will be recovered, even 90-98%. However, depending on the size of a small molecule, it is hard to determine if 20-80% of something smaller will pass through. Shape matters and size matters. In this example, if you had a 1 kDa small molecule and a 20 kDa, one may expect more of the 1 kDa to pass through and less of the 20 kDa, if any. One key can sometimes be to spin slower and longer. By doing this, more of the small may pass through.
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Can product UFC505008, Amicon® Ultra Centrifugal Filter, 50 kDa MWCO effectively concentrate the AAV virus sample ? Is it recommended to block the membrane before adding the sample as AAV strongly adhere to the column?
1 answer-
Yes, product UFC505008 ,Amicon Ultra Centrifugal filter is suitable for concentrating the AAV virus up to a certain point. However, to optimize the process and prevent aggregate formation, it is recommended to use larger volume Amicon Ultras (15 ml) and avoid over-concentration.
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My antibody solution contains 20% glycerol. Can I use this poduct to get rid of the glycerol? which dialysis buffer should be used?
1 answer-
Yes, Amicon products that have a 10 kDa NMWCO pore size are suitable for the removal of glycerol from antibody preparations. The protocol below was provided by one of our antibody suppliers - Atlas Antibodies. Note that this protocol is written for the 0.5 mL product UFC501096, Volumes and possibly spin times would have to be adjusted to accommodate the 15 mL Amicon products.
Glycerol can be removed using Amicon Centrifugal Filter Units, 10 kDa cutoff (Merck Millipore UFC501096).
The filter unit concentrates the antibody sample during centrifugation by allowing the buffer to flow through the membrane but stops any proteins above 10 kDa from passing.The antibody is then resuspended in a buffer of choice, thus allowing for buffer exchange. Consider testing the antibody +/- the glycerol cleanup, to see whether there is an improvement. Some antibodies perform better even if there is glycerol in the buffer.
PROCEDURE
1. Dilute the antibody to 400 μl with sterile PBS (or other buffer of choice).
2. Place the Amicon filter unit into the supplied collection tube.
3. Add 400 μl of diluted antibody to the filter unit. Close the lid.
4. Spin for 5-10 minutes at 14k*g or until 100 μl of the antibody sample remains in the spin column. If this has not been achieved during the first 5 min, then centrifuge for a few more minutes until only 100 μl remains.
Please note that the suggested time it takes to concentrate the sample down to 100 μl should be seen only as a guideline and will ultimately have to be determined by the user. For all spins, place the filter part of the filter unit and the elbow of the collection tube towards the centre of the rotor. All spins are performed at room temperature.
5. Discard the flow through.
6. Add 300 μl of sterile PBS (or other buffer of choice) to the filter unit and centrifuge again as outlined in step 4. Then discard the flow through.
7. Repeat step 6 four more times until the glycerol content is 0,02 %. 5-minute centrifugations are usually enough to reach 100 μl left in the filter unit at these steps. In the last round, centrifuge for 4 minutes until 100 μl is reached.
8. In new collection tube, place spin column upside down.
9. Spin for 2 min at 1k*g to collect buffer-exchanged antibody.Helpful?
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Hi , what simliar items do you have for this? I need 15ml centrifugal filters 50kda
1 answer-
Unfortunately, this is the only 15mL centrifugal filter with 50kDa MWCO offered. There are other products available at different MWCO or volumes such as the Corning Spin-X UF products, see CLS431490 (20 mL).
To discuss other options, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.
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