PC138

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

liquid, Calbiochem^®

• Synonym(e): Anti-Nuclear Factor ΚB
• NACRES: NA.43
• UNSPSC Code: 12352203

Eigenschaften

• Produktname: Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb, liquid, Calbiochem^®
• biological source: rabbit
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (ELISA ; Gel Shift ; Immunoblotting (1:500, chemiluminescence); Immunoprecipitation )
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 100

Beschreibung

Analysis Note

Positive Control
Daudi cells

Application

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.

Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogen

Human

a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Other Notes

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.

This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Physical form

In 5 mM sodium phosphate buffer, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable