OP93
Anti-BRCA1 (Ab-2) Mouse mAb (MS13)
liquid, clone MS13, Calbiochem®
Synonym(e):
Anti-Breast Cancer Susceptibility Protein
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
MS13, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität
human
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
do not freeze
Isotyp
IgG1
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... BRCA1(672)
Allgemeine Beschreibung
Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections.
Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.
Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Immunogen
Epitope: within amino acids 1-304
Human
recombinant, human BRCA1
Anwendung
Immunoblotting (2 µg/ml, chemiluminescence; see application references)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Hinweis zur Analyse
Positive Control
MCF7 cells
MCF7 cells
Sonstige Hinweise
Antibody should be titrated for optimal results in individual systems.
Scully, R., et al. 1996. Science272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.
Rechtliche Hinweise
Sold under license of U.S. Patents 5,753,441 and 6,162,897.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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S Fan et al.
Oncogene, 20(35), 4827-4841 (2001-08-25)
The tumor suppressor activity of the BRCA1 gene product is due, in part, to functional interactions with other tumor suppressors, including p53 and the retinoblastoma (RB) protein. RB binding sites on BRCA1 were identified in the C-terminal BRCT domain (Yarden
S Fan et al.
Oncogene, 20(57), 8215-8235 (2002-01-10)
Unregulated expression of wild-type BRCA1 (wtBRCA1) confers an altered phenotype in cultured human prostate cancer cells, characterized by chemosensitivity, susceptibility to apoptosis, decreased DNA repair activity, and alterations of key cell regulatory proteins. We now report that the expression of
Xiaolei Pan et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(29), E5940-E5949 (2017-07-05)
In the mammalian genome, certain genomic loci/regions pose greater challenges to the DNA replication machinery (i.e., the replisome) than others. Such known genomic loci/regions include centromeres, common fragile sites, subtelomeres, and telomeres. However, the detailed mechanism of how mammalian cells
Yong Xian Ma et al.
Oncogene, 22(1), 10-27 (2003-01-16)
The heat shock response is an evolutionarily conserved response to heat and other stresses that promotes the maintenance of key metabolic functions and cell survival. We report that exposure of human prostate (DU-145) and breast (MCF-7) cancer cells to heat
Jing Yang et al.
International journal of biological sciences, 17(12), 3239-3254 (2021-08-24)
Breast cancer susceptibility gene 1 (BRCA1) encodes a tumor suppressor that is frequently mutated in familial breast and ovarian cancer patients. BRCA1 functions in multiple important cellular processes including DNA damage repair, cell cycle checkpoint activation, protein ubiquitination, chromatin remodeling
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